Study Finds 4-HO-TMT is Active at the 5-HT2A Receptor

Researchers at CaaMTech, the University of Massachusetts Dartmouth, and Canopy Growth USA published an article in ACS Omega detailing the synthesis, crystal structure, and serotonin receptor activity of the aeruginascin metabolite 4-HO-TMT¹. They found that 4-AcO-TMT had a binding affinity at the four serotonin receptors 5-HT_1A, 5-HT_2A, 5-HT_2B, and 5-HT₃.

4-HO-TMT showed less binding affinity at 5-HT_2A than psilocin. However, the authors concluded that the K_i values were comparable (670.0 nM and 107.2 nM, respectively). Also, the data showed that 4-HO-TMT has a similar affinity for 5-HT_2B compared to psilocybin. The compound showed no activity at 5-HT₃.